MicroRNA-seq of MDA-MB-231 cells transduced with anti-ELOVL5 and control shRNAs

MiRNA isoforms (isomiRs) are single stranded small RNAs originating from the same pri-miRNA hairpin as a result of cleavage by Drosha and Dicer enzymes. Variations at the 5’-end of a miRNA alter the seed region of the molecule, thus affecting the targetome of the miRNA. MDA-MB-231 cells were transduced with shRNAs against ELOVL5 and luciferase genes. Small RNA sequencing was used to study the cleavage profiles of shRNAs. Sequencing was performed for two samples: MDA-MB-231 cells trancduced with shELOVL5 and MDA-MB-231 cells trancduced with shLuc.