Transcriptome profiles of P. aeruginosa treated with manuka honey and its key components

The aim of this study was to characterise the transcriptomic changes in P. aeruginosa PA14 following treatment with antimicrobial manuka honey and its key components (sugar, methyglyoxal, sugar + methylglyoxal) for 30 minutes at a subinhibitory concentration (0.5x MIC). mRNA profiling was conducted to gain mechanistic insights into the mode of action of antimicrobial manuka honey. mRNA profiles of mid-exponentially growing P. aeruginosa treated with manuka honey and its key components (sugar, methyglyoxal, sugar + methylglyoxal) at subinhibitory concentrations for 30 minutes were measured by RNA-sequencing, in triplicate, on an Illumina HiSeq4000 sequencer