RNA-seq to identify P-body associated mRNAs upon glucose starvation, Na+ and Ca2+ stresses

To examine mRNA species sequestered by P-bodies upon different stress conditions, we employed a modified crosslinking immunoprecipitation (cCLAP) technique to purify P-body associated mRNAs and analyzed by RNA-Seq. To compare, we also performed total RNA-Seq under the same stress conditions. Overall design: five biological independent replicates for P-body associated RNA-Seq, each consists of four conditions (control, glucose starvation, Na+and Ca2+) and three biological independent replicates for total RNA-Seq, each consists of four conditions (control, glucose starvation, Na+and Ca2+).