CIL: 50889, Saccharomyces cerevisiae (baker's yeast, budding yeast), Mixed population of S. cerevisiae cells. In Cell Image Library
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Heat shocked cells were cultured in YPD medium and high pressure frozen in a Wohlwend Compact 3 machine,
followed by a short freeze substitution protocol in 2% Uranyl acetate and embedded in HM20 resin. For the immuno-EM, samples were blocked for 2 hours in 0.8% BSA with 0.1% fish skin gelatine. A 1:10 dilution of the primary antibody ab19247 (Abcam) was used and a 1:20 dilution of the secondary antibody anti-Rabbit IgG 10 nm gold (Cat#25108, Electron microscopy sciences). Finally, samples were post fixed in 2.5% glutaraldehyde for 20 minutes. Sections of 70 nm thickness were then contrast stained with 2% Uranyl acetate and Reynold's lead
citrate. Pictures were aquired in a Tecnai T12 TEM with a Ceta CMOS 16M camera.
提供机构：
UC San Diego Library Digital Collections
创建时间：
2021-04-16
