Expression data from dorsal pallium Ctip2+ neuron at E14.5 and E16.5

Cell-based therapy is a promising treatment for neurodegenerative diseases such as Parkinson’s disease or cardiac disease. Similarly, reconstruction of the corticospinal tract by cell transplantation is expected as a treatment for stroke and degenerative disease. For efficient cell integration to the corticospinal tract after transplantation, the induction or selection of corticofugal projection neuron (CFuPN) is important. However, no CFuPN specific surface markers for the purification are known. Recently, miRNAs have been reported as alternatives to surface markers; microRNA responsive switch. In order to separate CFuPN at the axon extension stage, which is optimal for regenerative medicine, by using miRNA switches, we used microarrays to identify miRNAs that are specifically expressed in CFuPN during cerebral cortex V-VI layer formation. Corticofugal projection neuron at embryo were selected at successive stages of early development for RNA extraction and hybridization on Affymetrix microarrays. We sought to obtain homogeneous populations of embryos at each developmental stage in order to increase the temporal resolution of expression profiles. To that end, we made Ctip2-EGFP transgenic mouse then, surgically resect only dorsal pallium of E14.5 and E16.5 mouse embryo. By these methods, only corticofugal projection neuron at axon elongating stage can be purified.