Captive held Hippocampus erectus with and without mycobacteriosis evaluated for putative gene expression. Samples of head kidney used for RNA extraction, RNA-seq and transcriptome analysis.
SMRT sequencing was performed using a total of 5 ug of the genomic DNA to generate a 20 kb library according to the manufacturer's instructions. Subreads were obtained after size selection on the Blue
The library was prepared following the standard in situ HiC protocol for blood samples, using DpnII as the restriction enzyme. Standard circularization step was carried out, followed by DNA nanoballs