Bulk RNA-seq of intestinal organoids upon treatment with LXR agonist GW3965

Liver X Receptor (LXR) activation in intestinal epithelial organoids promotes their growth in vitro. To investigate the downstream effect of LXR activation in intestinal epithelial cells and identify potential pathways driving regeneration, we carried out an unbiased transcriptomic analysis of intestinal epithelial organoids stimulated with the LXR agonist GW3965. In detail, wild type small intestinal crypts were seeded in vitro and stimulated with vehicle (DMSO) or GW3965. After six hours, closing crypts (organoids) were collected for RNA extraction and were analyzed by RNA sequencing. Overall design: Small intestinal crypts were isolated from two C57BL/6J male mice and seeded in vitro in Matrigel and organoids growth media. DMSO (vehicle) or GW3965 (2uM) was added in the overlaying media. Crypts from each mouse were stimulated with both vehicle and GW3965 (n=2 in each experimental group). After 6 hours, organoids were collected and RNA extracted and submitted for RNAseq analysis.