Application of DENV RVPs to clinical serum samples.

Longitudinal serum samples taken at 3, 6, 12 and 18 months post-symptom onset from two primary DENV-1, one primary DENV-2, two primary DENV-3, and one secondary DENV-2 infection were obtained from naturally infected patients in Nicaragua. The serum representing secondary infection was derived from a patient that was last infected by DENV-2, as determined by RT-PCR and virus isolation. Each serum was used in neutralization assays against each DENV serotype. ‘<10’ (non-neutralizing) indicates failure of the serum to neutralize by at least 50%. Each sample was run once, in duplicate. DF, Dengue Fever; DHF, Dengue Hemorrhagic Fever. ND = not determined.