Comparative expression analysis of wild-type and OsCAld5H1-knockout rice plant using Agilent Rice Gene Expression 44k Array

Knockout of a gene encoding coniferaldehyde 5-hydroxylase (OsCAld5H1) leads to modulate increase guaiacyl (G) lignin aromatic composition in rice. We compared the global gene expression profiles of wild-type and OsCAld5H1-knockout mutants by microarray to examine the possible effects of OsCAld5H1 disruption on gene expression. We subsequently applied the up- and down-regulated gene sets to gene ontology term enrichment analysis. The results demonstrated that no GO biological process categories enriched significantly, indicating that minimal perturbation of gene expression were caused by OsCAld5H1-knockout. Gene expression in wild-type and OsCAld5H1-knockout rice internode was measured at the heading stage. Three biological replicates were prepared for each genotype.