Transcriptome wide gene expression analysis with inducible silencing of YY1 in AW8507 oral cancer cells. Transcriptome wide gene expression analysis with inducible silencing of YY1 in AW8507 oral cancer cells

In order to study the role of the transcription factor YY1 in oral cancer progression, transcriptome wide gene expression analysis was performed with inducible silencing of YY1 in a grade III, tongue derived oral cancer cell line, AW8507 (Established and characterized by Tatake RJ et al. 1990) stable for tet-ON-shYY1. Overall design: AW8507 cells were transfected with TRIPZ-tet-ON-shYY1 plasmid and tet-ON-shYY1 stable cells were obtained by selection with puromycin followed by FACS for RFP positivity. The AW8507 cells stable for tet-ON-shYY1 were treated with doxycycline to induce tet-ON system, therefore expression of shRNA against YY1, which resulted in silencing of YY1 mRNA (Confirmed by qRT-PCR). Total RNA was isolated from control and doxycycline group and transcriptome wide gene expression changes were analyzed via microarray based approach.