Transcriptional analysis of naïve and central memory CD4 and CD8 T cells from healthy subjects treated with IL-27

To examine the broad impact of IL-27 on human T lymphocytes, we performed a microarray analysis assessing >20,000 well annotated genes on purified naïve (CD45RA+CD45RO-CCR7+) and central memory (CD45RA-CD45RO+CCR7+) CD4+ and CD8+ T cells from three healthy donors that were activated in vitro (plate bound anti-CD3 and soluble anti-CD28) in the presence or absence of human recombinant IL-27 (100 ng/mL). Our goal was to investigate the impact of interleukin-27 on the gene expression profil of human CD4 and CD8 T lymphocytes. Naive and central memory CD4 and CD8 T cells from 3 healthy donors were sorted and then stimulated for 3 days with αCD3-αCD28 in the presence or not of IL-27 (100 ng/mL). RNA was purified and transcriptome-wide analysis of gene expression was performed by Genome Québec using the Clariom S HT array.