Figure S1 - The Influence of Oxygen on [NiFe]–Hydrogenase Cofactor Biosynthesis and How Ligation of Carbon Monoxide Precedes Cyanation

Cyanide ligand scrambling upon prolonged incubation with 13CO. At 1 mbar and incubation times +1 h (room temperature, ambient light) 13CO not only binds to Fe(I)–CO but continuously replaces the ‘natural’ 12CO ligand of the Fe(II)–(CN)2CO cofactor whose spectrum serves as background in fig. S1. The black trace shows 12CO binding to a sample of HypCDMC as discussed in the main script. The red trace (13CO binding) gives rise to derivative–shaped signals in the Fe(II)–CN– region (here 2050–2100 cm−1). Both spectra were recorded under comparable conditions.
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