Comparison of Bruker Biotyper (R) and Vitek (R) MS matrix-assisted laserdesorption/ionization–time-of-flight massspectrometry platforms for the identificationof filamentous fungi - Supplemental Table 1

Aims: To evaluate the performance of twomatrix-assisted laser desorption/ionization–time-of-flight mass spectrometry platforms to identify molds isolated from clinical specimens. Methods: Fifty mold isolates were analyzed on Bruker Biotyper R ? and Vitek R ? MS platforms. Two Bruker Biotyper extraction protocols were assessed alongside the US FDA-approved extraction protocol for Vitek MS. Results: The Bruker Biotyper modified NIH-developed extraction protocol correctly identified more isolates than Bruker’s protocol (56 vs 33%). For species in the manufacturers’ databases, Vitek MS correctly identified 85% of isolates, with 8% misidentifications. The Bruker Biotyper identified 64%, with no misidentifications. For
isolates not in the databases, the Bruker Biotyper did not misidentify any, and VitekMSmisidentified 36%. Conclusion: Both the VitekMS and Bruker Biotyper accurately identified the fungal isolates, however Vitek MS was more likely to misidentify isolates than the Bruker Biotyper.

研究目的：旨在评估两种矩阵辅助激光解吸/电离-飞行时间质谱平台在识别从临床样本中分离出的霉菌方面的性能。研究方法：在Bruker Biotyper R ? 和 Vitek R ? MS平台上分析了五十个霉菌分离株。在评估美国食品药品监督管理局（US FDA）批准的Vitek MS提取方案的同时，还评估了两种Bruker Biotyper提取方案。研究结果：Bruker Biotyper改进的NIH开发提取方案正确识别的分离株数量多于Bruker的方案（56%对33%）。对于制造商数据库中的物种，Vitek MS正确识别了85%的分离株，误识别率为8%。Bruker Biotyper识别了64%，无误识别。对于数据库中未收录的分离株，Bruker Biotyper未出现误识别，而Vitek MS误识别率为36%。研究结论：Vitek MS和Bruker Biotyper均准确识别了真菌分离株，然而，与Bruker Biotyper相比，Vitek MS误识别分离株的可能性更高。