Mount Sinai Hospital (MSH, Toronto, CANADA) pelvic pouch microbiome study

This study involved evaluation of the tissue associated microbiome of the ileal pouch following surgery for ulcerative colitis (UC) or familial adenomatous polyposis (FAP). Individuals were recruited, with biopsies taken from the ileal pouch and the pre-pouch ileum, microbial DNA was extracted and sequenced using 454 pyrosequencing. Total bacterial community structure and abundance were evaluated to determine which changes were characteristic of inflammatory phenotypes including pouchitis (inflammation of the ileal pouch) and a Crohn's disease-like phenotype.]]>
Inclusion Criteria Patients having undergone ileal pouch-anal anastomosis for ulcerative colitis (UC) or familial adenomatous polyposis (FAP) with ileostomy closure at least one year prior to study enrollment Sufficient clinical information is available to classify patients into post-surgical outcome groups Patients willing to undergo study pouchoscopy with biopsy Exclusion Criteria Pre-surgical diagnosis of Crohn's disease < 18 years old ]]>