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StitchR: Ribozyme-activated mRNA Trans-ligation and Translation. StitchR: Ribozyme-activated mRNA Trans-ligation and Translation

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NIAID Data Ecosystem2026-05-02 收录
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https://www.ncbi.nlm.nih.gov/bioproject/PRJNA1164324
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We report that ribozyme cleavage of two separate mRNAs activated their scarless trans-ligation and translation into full-length protein in eukaryotic cells, a process that we named StitchR (for stitch RNA). Overall design: StitchR 3.0 Nt-GFP and Ct-GFP AAV1-serotyped virus particles were generated and used to co-transduce HEK293T cells. Cells were harvested 48 hours post-transduction.
创建时间:
2024-09-23
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