Systematic Expression Profiling of the Mouse Transcriptome Using RIKEN cDNA Microarrays. Mus musculus

The number of known mRNA transcripts in the mouse has been greatly expanded by the RIKEN Mouse Gene Encyclopedia project. Validation of their reproducible expression in a tissue is an important contribution to the study of functional genomics. To assign absolute expression values for mouse transcripts, we applied the E17.5 reference sample to CAGE (cap analysis of gene expression) and expressed sequence tag (EST) high-throughput tag sequencing. We add a reliability index named SRED (Spot Reliability Evaluation Score for DNA microarrays) for each spot. The SRED scores represent the probability that the calibrated gene expression level from a DNA microarray would be less than a factor of 2 different from that of quantitative real-time polymerase chain reaction assays whose dynamic quantification range is treated statistically to be similar to that of the DNA microarray.