Additional file 5 of Microbial community characterization of shrimp survivors to AHPND challenge test treated with an effective shrimp probiotic (Vibrio diabolicus)

Additional file 5: Table S1. Primers sequences used in the study. AP4 (1 and 2) are for the detection of Pir-VP genes. Vpir A is for the detection of Pir-A gene while Vpir B is for the amplification of Pir-B gene. Length represents the length of the amplicon generated by each pair of primers. Table S2. ILI’s genome protein annotation using Prokka against the NCBI’s database. Table S3. ILI’s large plasmid proteins predicted by Prokka against the NCBI’s database with their corresponding functions and ID. The number of ASVs detected per sample is plotted on the axis of Observed OTUs. Function is assigned when hits with an e-value < 1e- 06 were obtained. Table S4. Genomes used to for genomic similarity analysis. The genomic identity between ILI and the selected genome is shown in the last column of the table. Table S5. GenBank accession numbers and genomic characteristics of genomes used for the phylogenetic reconstruction of the ILI strain. Table S6. DDH, ANIb and TETRA values. In silico DNA-DNA hybridization (DDH) was calculated only between the ILI strain and the other evaluated strains. ANIb and TETRA were calculated for all possible comparisons. A heatmap representation of the values can be observed on Figure 3. Table S7. Virulence factors identified in the secretion systems of the strain V. diabolicus ILI. They were compared with the genomes of the V. parahaemolyticus RIMD 2210633 strain, the V. parahaemolyticus NCKU TN S02 strain, the V. parahaemolyticus BA94C2 strain and the V. diabolicus FDA 105 strain. The table includes the respective name of the genes for each genome, the gene product, the percent identity, and the percent coverage. Comparisons based on percentage of identity and coverage included are shown in key color.