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Data and code for: The Effects of Molting on the Expression of Insulin and Growth Factor Receptors in the Crustacean Molting Gland (Y-Organ)

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Zenodo2026-10-30 更新2026-11-05 收录
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Salmon quantifications, sample metadata and analysis code for the study of receptor tyrosine kinase (RTK) expression across the molt cycle in the Y-organ of the blackback land crab, Gecarcinus lateralis, and the green shore crab, Carcinus maenas. Files: glat_salmon_quant.tar.gz: Salmon output for 115 G. lateralis Y-organ RNA-seq libraries, 54 from the multiple leg autotomy (MLA) experiment and 61 from the eyestalk ablation (ESA) experiment. The analysis uses the 59 ESA males; the 2 female libraries are included for completeness. Each library has quant.sf, cmd_info.json, lib_format_counts.json and aux_info/meta_info.json. cmaenas_salmon_quant.tar.gz: the same files for 50 C. maenas libraries (25 Y-organ, 25 central nervous system), quantified from the reads of Oliphant et al. (2018), NCBI BioProject PRJNA400568. manifest.tsv: size and MD5 checksum of every file inside the two archives. samples.tsv and cmaenas_samplesheet.tsv: sample metadata (experiment and molt stage for G. lateralis; crab, tissue and molt stage for C. maenas). yo-rtk-molt-expression_af93c93.tar.gz: snapshot of https://github.com/invertome/yo-rtk-molt-expression at commit af93c93, with the analysis pipeline (dge_panel_pipeline v1.0.6), configuration files, result tables and figures. RTK_expression_Supplementary_Material.pdf: the Supplementary Material of the article (Figures S1 to S5, Tables S1 and S2). To rerun the analysis, unpack the repository archive, extract both quantification archives inside its top directory (they unpack into data/glat/salmon_output/ and data/cmaenas/salmon_output/), and follow the README. Raw G. lateralis reads are in the NCBI Sequence Read Archive under BioProject PRJNA1553483. Data are licensed CC BY 4.0. The code in the repository archive is licensed under the MIT License (its LICENSE file).

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2026-10-30
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