微电极阵列监测谷氨酸调节神经干细胞的神经活性

本研究制作了一个60通道微电极阵列（MEA），用于监测大鼠神经干细胞分化神经元的神经尖峰和局部场电位（LFP）。神经元生长在MEA表面以检测神经信号。实验中使用谷氨酸（Glu）调节神经活动。为了提高检测性能，在微电极位点表面修饰了铂纳米颗粒。使用MEA记录谷氨酸刺激的神经尖峰和LFP。在正常状态下，平均尖峰振幅约为70μV。在Glu调制下，从70μV到90μV的尖峰幅度增加了29%。在Glu调制下，发射频率从4.01Hz提高到6.8Hz，提高了69%。在0–10 Hz频带内，通过Glu调制，LFP功率从正常状态下的326μW增加到617μW。数据分析表明，Glu调制刺激的神经活动在高时空分辨率下被实验记录下来。这些结果可能提供一种新的神经元检测方法，以及进一步了解神经干细胞刺突放电及其相关机制

This study fabricated a 60-channel microelectrode array (MEA) for monitoring neural spikes and local field potentials (LFP) of neurons differentiated from rat neural stem cells. Neurons were cultured on the MEA surface to detect neural signals. Glutamate (Glu) was used to regulate neural activity in the experiments. To enhance detection performance, the surfaces of the microelectrode sites were modified with platinum nanoparticles. MEA was utilized to record neural spikes and LFP evoked by glutamate stimulation. Under normal conditions, the average spike amplitude was approximately 70 μV. Under Glu modulation, the spike amplitude increased by 29%, rising from 70 μV to 90 μV. Under Glu modulation, the firing frequency increased by 69%, from 4.01 Hz to 6.8 Hz. In the 0–10 Hz frequency band, the LFP power increased from 326 μW under normal conditions to 617 μW after Glu modulation. Data analysis showed that neural activities evoked by Glu modulation were experimentally recorded with high spatiotemporal resolution. These results may provide a novel neuronal detection approach and further insights into the spike firing of neural stem cells and their associated mechanisms.

搜集汇总

背景与挑战

背景概述

该数据集包含使用60通道微电极阵列（MEA）监测大鼠神经干细胞分化神经元在体外神经活动的实验数据，重点研究了谷氨酸（Glu）对神经电信号（如神经尖峰和局部场电位）的调节作用。实验数据显示，谷氨酸显著增加了神经尖峰的幅度和放电频率，以及局部场电位的功率。