File S1 - Metarhizium anisopliae Pathogenesis of Mosquito Larvae: A Verdict of Accidental Death

Figure S1-S2, Table S1, Text S1-S5. Figure S1. Limited antioxidant activity in mosquito larvae exposed to M. anisopliae. Activity of mosquito larvae exposed and not exposed to conidia of M. anisopliae. (A) Reactive oxygen species (ROS) generation, and activity of (B) MDA (lipid peroxidation), (C) catalase, (D) Superoxide dismutase (SOD),and (E) glutathione-S-transferase (GST). Data presented as mean ± (SEM) (Two-way ANOVA, **-pFigure S2. LCMS chromatogram showing no detectable Metarhizium anisopliae destruxin in Aedes aegypti larval extracts. Table S1. Metarhizium anisopliae and Aedes aegypti loci used for expression analysis. Text S1. Cryo-SEM. Text S2. Analysis of destruxins. Text S3. Enzyme and enzyme inhibitor assays. Text S4. Transcript quantification of insect and fungus-derived genes. Text S5. ROS production, lipid peroxidation and antioxidant system activity. (DOCX)