Increased expression of Fmrp-target genes in fmr1-/- zebrafish.

A. The full mRNA sequence of the fmr1 gene, including the CDS (black bars) and UTRs (white bars). A single C-to-T mutation at position 113 results in a premature stop codon and truncated protein (gray bars). B-D. Whole-mount ISH assays show the spatial expression of mtor, sash1, and tln1 in 6 dpf WT larvae. Fb, forebrain; Mb, midbrain; Hb, hindbrain. E. Relative mRNA expression of mtor, sash1, and tln1 in 6 dpf fmr1-/- (grey bars) and WT larvae (white bars). Values are represented as means ± SEM (*pt-test assuming unequal variances). F. Western blots show an approximate five-fold increase in the expression of mTor protein levels in fmr1-/- zebrafish brain tissue.