Comparison of performance of different methods for assaying <i>B. burgdorferi</i> viability.

1Number of cells per 100 µl culture. Linear regression analysis (least squares regression method) was used to determine the detection limit of the assays. No linear-relationship (r <0.9) between cell numbers and corresponding measurement value was observed when the cell number is less than the detection limit. The values for linear regression analysis are the mean of three replicates. 2Percentage of relative error from three time measurements. 3Test time required for completing a 96-well plate assay. 4ND: Not determined, due to weak signal of cells and/or strong background signal from BSK-H medium. 5BSK-H medium was removed by washing prior to LIVE/DEAD BacLight assay. 6Samples were stained with LIVE/DEAD BacLight assay. Comparison of performance of different methods for assaying B. burgdorferi viability.