Reduced accumulation of antigen-specific CD8+ T cells in Hvem−/− mice.

WT or Hvem−/− mice were orally infected with 1×109 LM-OVA and the amount of antigen-specific CD8+ T cells was measured. A- Percentage and absolute number of OVA-specific CD8+ T cells in splenocytes isolated from WT or Hvem−/− mice, 7 days p.i. B- IFNγ production by WT or Hvem−/− splenic CD8+ T cells after ex vivo restimulation with OVA257–264 peptide. The percentage of OVA-specific CD8+ T cells in the MLN (C) and IEL (D) of WT and Hvem−/− mice was also calculated, 7 days after infection. Data shown correspond to 6–9 mice per group, pooled from two independent experiments. E- Percentage and absolute number of memory OVA-specific CD8+ T cells in the spleen of WT and Hvem−/− mice, 70 days p.i. F- IFNγ production by OVA-specific memory CD8+ T cells isolated from the spleen of WT and Hvem−/− mice 70 days p.i. and restimulated ex vivo with OVA-peptide. Data were pooled from at least two independent experiments, each one performed with 5–7 mice per group.