George E. Palade (2011) CIL:7587, Rattus, hepatocyte. CIL. Dataset

This image shows the heterogeneity in the content of lysosomes near the plasma membrane between two hepatocytes and near the bile canaliculus (not visible in this field). Some of the lysosomes contain residues resulting from partial digestion of lipids and some of these are more electron dense than others. Not only does the content of lysosomes vary depending on the cell type, but also physiological conditions, and even amongst lysosomes within an individual cell. Also in the field are mitochondria, rough endoplasmic reticulum, and the cell membrane. This image is part of a set of two; both images are identical, but on one of the twins, organelles are labeled by name.

Small blocks of liver tissue were fixed in 4% osmium tetroxide in 0.1 M cacodylate buffer pH 7.4 for 2 hours. The tissue was dehydrated and embedded in Epon. Sections were stained for 1 minute with ethanolic uranyl acetate and then alkaline lead citrate for 5 minutes.

Original resource was provided as a 3.25 x 4 inch lantern slide by George E. Palade; original is in the Palade Collection, University of California, San Diego. Digitization process: Lantern slide scanned at 1200 dpi in TIFF format, labeled in Photoshop then reduced to 600 dpi TIFF file (3500 x 2705 pixels) prior to conversion to JPEG2000 format.

本图展示了邻近质膜与胆管之间的肝细胞之间溶酶体的异质性内容。部分溶酶体含有由脂质部分消化产生的残留物，其中一些残留物的电子密度高于其他。溶酶体的内容不仅随细胞类型而异，还受生理条件的影响，甚至在单个细胞内的溶酶体之间也存在差异。图中还可见线粒体、粗糙的内质网和细胞膜。此图像是两组图像中的一幅；两组图像完全相同，但在其中一幅图像中，细胞器以名称进行了标注。

小块肝脏组织在0.1 M草酸盐缓冲液pH 7.4中用4%的四氧化锇固定2小时。组织脱水并嵌入环氧树脂中。切片用乙醇铀酸醋酸染料染色1分钟，然后用碱性铅铬酸盐染料染色5分钟。

原始资源由George E. Palade提供，以3.25 x 4英寸的幻灯片形式存在；原始资料存放在加州大学圣地亚哥分校的Palade收藏中。数字化过程：幻灯片以1200 dpi分辨率扫描为TIFF格式，然后在Photoshop中进行标注，最后将分辨率降至600 dpi的TIFF文件（3500 x 2705像素），并转换为JPEG2000格式。