Selected PK parameters and clinical signs in ferrets challenged with influenza A viruses (Study 1).

a OP dose was calculated based on free-base oseltamivir molecular weight.b Values are mean ± SD. All PD parameters were from morning observations.c Each ferret’s body temperature was measured by subcutaneous implantable encapsulated microchips (IPTT-300, Bio Medic Data Systems, US), which was recorded for 3 days before virus inoculation. The body temperature change was calculated for each ferret individually as an increase or decrease of its temperature on day 0 before virus inoculation. The values are averages for six animals per group (°C) ± SD. The average temperature decrease is indicated by minus. Positive average values indicate that the temperature increased. The number of ferrets per the total number of animals in that group that had at least a 1°C increase in temperature from baseline is shown in parenthesis.d The weight change was calculated for each ferret individually as a percentage of its weight on day 0 before virus inoculation. The values are averages for six animals per group (%) ± SD. The average weight loss is indicated by a minus sign. Positive average values indicate that animals were gaining weight. The number of ferrets per total number of animals in that group that had more than a 5% loss of initial weight on day 5 p.i. is shown in parenthesis.e The reported value is the mean morning activity score ± standard deviation observed on days 1–5 after inoculation on day 0. A score of zero indicated no nasal symptoms and a fully playful ferret. A score of one indicated nasal rattling or sneezing and a ferret that responded to play overtures but that did not initiate play. A score of two indicated nasal discharge and a ferret that was alert but not playful. A score of three indicated that mouth breathing was necessary and the ferret was not playful or alert.f Blood samples were not collected as OP was not administered.AUCss12h = steady-state 12-h area under the plasma concentration-time curve calculated from the population PK model individual fit CL for each animal: AUCss12h = D×CL; CL = clearance; OC = oseltamivir carboxylate; OP = oseltamivir phosphate; PD = pharmacodynamic; p.i. = post-infection; PK = pharmacokinetic; SD = standard deviation; TCID50 = 50% tissue culture infectious dose.Selected PK parameters and clinical signs in ferrets challenged with influenza A viruses (Study 1).