The transcription factor, α1ACT, acts through a microRNA network to regulate neurogenesis and cell death during neonatal cerebellar development

To investigate whether α1ACT acts through a microRNA network, we studied α1ACT-associated simultaneous miRNA:mRNA expression transcriptome profiles during cerebellar development in a context-dependent manner, using miRNA-seq paired with previously reported RNA-seq. Examination the miRNA-seq results between pc12 cell lines stably expressing blank vector and α1ACT; pairing the differentially expressed miRNA with previously reported DEGs via miRWALK to investigate the α1ACT mediated miRNA regulation network. All outcomes were validated via qRT-PCR