Identification of RNA target of Matrin3 and PTBP1 in neuroblastoma cell line SH-SY5Y by PAR-CLIP. Homo sapiens

RNA-binding proteins (RBPs) act on each target separately or the same target cooperatively or competitively. The aberrance in RBPs leads to neurological disorders such as amyotrophic lateral sclerosis (ALS). Matrin3 is highly conserved DNA/RNA binding protein whose point mutations are found in familial ALS patients. However, the function and target of Matrin3 in neuronal cells remain unclear. Here, we report the transcriptome-wide binding sites of Matrin3 in neuroblastoma SH-SY5Y cell lines using photoactivatable-ribonucleoside-enhanced crosslinking and immunoprecipitation (PAR-CLIP). These data will provide us first step to understand pathological mechanism of ALS.