Effects of glucose on human islet insulin biosynthesis.
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Islet in groups of 20 were cultured for 24 hours at 5.6 or 20 mM glucose and then labeled with 3H-leucine for two hours in a KRBH buffer containing BSA and 20 mM glucose. Insulin was immunoprecipitated with guinea pig anti-insulin antibodies and protein A sepharose, and total proteins were precipitated with trichloroacetic acid. Incorporated radioactivity was quantified by liquid scintillation counting and results are means ± SEM for 4 observations.*denotes p
创建时间：
2015-12-02
