Read me file and data for the study of the link between mitochondrial DNA and metabolic rate.docx

Read me file and data files for a project with the aim to optimize a quantitative PCR (qPCR) for the mitochondria cytochrome c oxidase subunit I gene, use the method as a proxy for mitochondrial DNA content, and explore if young trout with lower metabolic rates have a reduced mitochondria content. We extracted liver and muscle tissue of controls (n=19) and warm (n=20) incubated juvenile brown trout and successively optimized a qPCR for mitochondrial DNA.