Categorization of proteins based on fold change, R-squared values and major expected contributing source of origin.

In order to explore how different proteins were affected by the rostro-caudal gradient, we used the fold change and R-squared values of the proteins quantified with SID-MRM and iTRAQ to categorize the proteins into the three categories: affected by the RCG, unaffected by the RCG and uncertain. The fold change was calculated between the 1-2nd and 44-45th mL of CSF (referred to as RCG point 1 and 7), and the classification was based on the criteria from Table 1.The proteins are also categorized into groups based on the major expected contributing source of origin, with Uniprot as the primary reference unless otherwise stated. See Table S5 for details. The asterisk (*) marks conflicting results: affected + unaffected = uncertain; affected + uncertain = affected; unaffected + uncertain = unaffected. Proteins only quantified in the SID-MRM study is marked with a.