Data from: Nerve-dependent regulation of H3K27me3 during the induction of patterning competency in regenerating axolotl limb cells

Limb regeneration in the Mexican axolotl requires the dedifferentiation of
mature limb cells into progenitor cells known as blastema cells. Blastema
cells become responsive to the signals that define the blueprint, or
pattern, of the regenerating cells organizing into complex limb
structures. Signaling downstream of the limb nerves plays a significant
role in inducing patterning competency; however, the foundations of this
process remain poorly understood. Studying patterning competency in
amputated axolotl limbs is challenging because it occurs within the
context of an intricate network of signals and tissue interactions that
are activated simultaneously during regeneration. Here, we develop a
simplified limb injury model assay called the competency accessory limb
model (CALM), which we used as a platform to characterize the specific
timing of competency induction and maintenance and identify the H3K27me3
chromatin signatures associated with this cellular state. We also
discovered that a combination of FGF and BMP ligands is sufficient to
induce patterning competency in limb wound cells and identified the ErBB
pathway among the regulatory epigenetic targets downstream of these
signals.