Mycobacterium fragae strain:HF8704 Genome sequencing and assembly. Mycobacterium fragae strain:HF8704

Cells appear as short rods, typically acid–alcohol-fast and not motile. Colonies are smooth and unpigmentated. Growth is obtained in LJ in 3–4 weeks at temperatures between 30 and 37 °C. The biochemical profile is characterized by positivity for tellurite reduction and heat-stable catalase (68 °C) and negativity for niacin accumulation, nitrate reduction, semiquantitative catalase (>45 mm), urease, arylsulfatase activity at 3 days and Tween 80 hydrolysis. Growth does not occur on MacConkey agar without crystal violet and no inhibition is observed on media containing TCH, p-nitrobenzoic acid or hydroxylamine. The antimicrobial pattern is characterized by susceptibility to clarithromycin, ethambutol, amikacin, linezolid, ciprofloxacin, streptomycin, doxicyclin, moxicyclin, rifampicin and sulfamethoxazole. The HPLC of mycolic acids allows a clear distinction from closely related species. The sequences of 16S rRNA, hsp65 and rpoB genes are unique.