Estimation of RNA positive Legionella pneumophila vesicles

Evaluation of RNA containing extracellular vesicles ariving from Legionella pneumophila
Conclusion:
Approximately 30% of the examined Lp-vesicles contain detectable amounts of RNA labeled by Syto(R)RNA-Select.
Notes:
Due to small size of the vesicles, with diameters smaller than the wavelength of the lasers, it makes it quite challenging to analyze and to distinguish them from the background noise. Therefore, in particular for our experiments with Syto®RNA-Select we triggered the gates threshold to reduce the background noise even though we are aware that we are only able to detect very large vesicles or MV-agglomerates which however frequently appear during the purification process. Characterization of the Lp-MVs by flow cytometry