Modeling pH and temperature effects as climatic hazards in Vibrio vulnificus and Vibrio parahaemolyticus planktonic growth and biofilm formation

This study aimed to better understand the potential effects of temperature and pH on V. vulnificus and V. parahaemolyticus planktonic and biofilm growth. Vibrio strains were grown in triplicate at 25°C, 30ºC, and 37ºC in 96 well plates containing Modified Seawater Yeast Extract modified with CaCl2 at pH’s ranging from 5 to 9.6. AMiGA software was used to model growth curves using Gaussian process regression. The effects of temperature and pH were evaluated using randomized complete block analysis of variance, and V. parahaemolyticus and V. vulnificus growth rates were modeled using the interpolation fit on the MatLab Curve Fitting Toolbox. Different optimal conditions involving temperature and pH were observed for planktonic and biofilm Vibrio growth within- and between-species. This study showed that temperature and pH factors significantly affect Vibrio planktonic growth rates and Vibrio parahaemolyticus biofilm formation. Vv_Planktonic_SPSS_All: All V. vulnificus strains for planktonic growth rates and statistics Vv_Biofilm_SPSS_All: All V. vulnificus strains for biofilm growth rates and statistics Vp_Planktonic_SPSS_All: All V. parahaemolyticus strains for planktonic growth rates and statistics Vp_Biofilm_SPSS_All: All V. parahaemolyticus strains for biofilm growth rates and statistics