5'-NAD-RNA identification in E. coli BW25113 wt, nudC and csdA knockouts

In the present study we applied NAD captureSeq to charecterize the 5'-NAD-RNA profile of E. coli BW25113 wt strain as well as nudC and csdA knockouts. The data obtained showed that both knockouts resulted in an increased number of identified 5'-NAD-RNA species, thus suggesting that CsdA might add a potentially new layer of control on NAD-epitranscriptomic landscape. Overall design: 5'-NAD-RNA identification in E. coli BW25113 wt, nudC KO and csdA KO strains. 3 Biological replicates for ADRPC+ and 2 biological replicates for ADRPC- libraries.