(Epi)genetic regulation of zebrafish intestinal development

The goal of the experiment was to characterize the wild type larval intestine in zebrafish. Tg(cldn15la:GFP) intestine-specific transgene was used to ease dissections. RNAseq was performed at 5, 7, 9 dpf on wild type dissected intestines to characterize the transcriptome. ChIPseq was performed at 5, 7, 9 dpf on wild type dissected intestines to check the intestinal presence of active (H3K4me3) and repressive (H3K27me3) chromatin marks and to correlate their presence with intestinal gene expression. Overall design: RNAseq of wild type dissected Danio rerio intestines, pools of 10 intestines in triplicates at 5, 7, and 9 dpf. ChIPseq of wild type dissected Danio rerio intestines, pools of 25 intesines (+5 set aside for input DNA) in duplicates at 5, 7, and 9 dpf for H3K4me3 and H3K27me3.