Mycobacterial rhomboid-encoding genes display differences in complementing aarA.

This table summarizes phenotype rescue ability in XD37.A by mycobacterial rhomboid-encoding genes. With the exception of MAP2426c and MAP2425c, all genes encoding the orthologs of “rhomboid protease 2” fully restored AarA activity (i.e. the golden yellow pigment of colonies, cell morphology, growth on MacConkey agar and the putative signal). MSMEG_5036 was the only “rhomboid protease 1” encoding gene that behaved like genes encoded by “rhomboid protease 2” but comparatively weaker. However, all mycobacterial rhomboid-encoding genes (except those of those of MAP) strongly activated production of the extracellular putative signal. Species/strains from which rhomboids were cloned are indicated in brackets (MTB H37Rv, MTB clinical isolates [BN44], M. bovis BCG, M. bovis cattle strain [JN55], M. avium clinical isolate [SU-36800], MAP and M. smegmatis [SMR5]).aP. stuartii wild type strain;bP. stuartii rhomboid deficient strain;cP. stuartii rhomboid deficient strain complemented with aarA (wild type gene);eTwo hypothetical rhomboid gene fragments (encoding MAP2426c and MAP2425c) cloned in-frame;-, not applicable.