Significance of changes in protein ratios.

Milk protein expression was analysed in wild-type mice [+/+] (n = 3), heterozygous mice [+/−] (n = 6) and α-casein deficient mice [−/−] (n = 5). Expression was quantified by densitometric scanning of protein gels and correlation of the net intensities with a molecular weight marker of known concentration. The protein ratios of α-casein to albumin (acas/alb.), α-casein to β-casein (acas/bcas), β-casein to γ-casein (bcas/gcas), β-casein to albumin (bcas/alb.) and γ-casein to albumin (gcas/alb.) were determined. The data were analysed by ANOVA for one way comparisons. The p values obtained for the different comparisons are shown. P values in bold print are below the cut off points of 0.01 or 0.001 (as indicated).