The role of BAF60C in type 2 diabetes——scRNA-seq

This research identify reduced expression of BAF60C in diabetic islets and demonstrate that altered ß-cell BAF60C levels strongly influence metabolic homeostasis. Genetic loss of BAF60C in ß-cells exacerbates hyperglycemia and islet dysfunction under metabolic stress, whereas ß-cell–specific restoration of BAF60C produces protective effects. These findings establish BAF60C as an important regulator of ß-cell integrity and disease progression in T2D. Overall design: We used scRNA-Seq to assess islets isolated from beta-cell-specific ablation (BcBKO) mice. Islets for scRNA-Seq data were isolated from flox/flox and BcBKO Pdx1-cre mice, then dissociated into single cells for sequencing. You can find detailed information on sample collection and data analysis pipeline in our manuscript.