BIO407 Group1 Widefield images of DFX treated and untreated cells

# REMBI metadata ## Study:This data is part of the practical course BIO407 2026 at the University of Zurich, titled 'Advanced Microscopy: From preparation to data and visualization'.The effects of Deferasirox (DFX) on mitochondria morphology were examined using different microscopy techniques. ## Study Component:Job: Widefield Microscopy Task: 5Using a widefield microscope imaging was performed on Deferasirox treated and untreated cells. ## Biosample:COS-7 cells ## Specimen:Four different conditions:-Control 1: No treatment-Control 2: 5h DMSO treatment-Condition 1: 3h 200uM DFX treatment-Condition 2: 5h 200uM DFX treatment Cells were fixed with 4% PFA, 0.05% GTA, 0.5% Saponin in PHEM.Cells were labeled with DAPI, TOMM20-Alexa488, Tubulin-Alexa568, SiR-Actin. ## Image Acquisition:The images were acquired using a LeicaTHUNDER Imager 3D Live Cell on the Leica DMi8 platform.Acquired channels:Channel 1: DAPI, 390nm excitation, 460/80nm emissionChannel 2: Alexa488-TOMM20, 475nm excitation, 535/70nm emissionChannel 3: Alexa568-Tubulin, 555nm excitation, 590/50nm emissionChannel 4: SiR-actin, 635nm excitation, 642/80nm emissionObjective: 40x LEICA HC PL APO CORR air objective, 0.95NA ## Image Data: BIO407_G1_WF_Thunder_task_5.lif/BIO407_G1_WF_Thunder_task_5_40x/Control 1 No treatmentBIO407_G1_WF_Thunder_task_5.lif/BIO407_G1_WF_Thunder_task_5_40x/Control 2 5h DMSO treatmentBIO407_G1_WF_Thunder_task_5.lif/BIO407_G1_WF_Thunder_task_5_40x/3 hours DFX_1 3h DFX treatmentBIO407_G1_WF_Thunder_task_5.lif/BIO407_G1_WF_Thunder_task_5_40x/3 hours DFX_2 3h DFX treatmentBIO407_G1_WF_Thunder_task_5.lif/BIO407_G1_WF_Thunder_task_5_40x/5 hours DFX_1 5h DFX treatmentBIO407_G1_WF_Thunder_task_5.lif/BIO407_G1_WF_Thunder_task_5_40x/5 hours DFX_2 5h DFX treatment