Exosomes from microglia attenuate photoreceptor injury and neovascularisation in an animal model of retinopathy of prematurity

Total RNAs from exosomes was used for miRNA library preparation and sequencing.The preparation and sequencing of exosome miRNA were performed by Ribobio (Guangzhou, China). First, total RNA samples were fractionated by using 15% Tris-borate-EDTA (TBE) polyacrylamide gel (Invitrogen), then small RNAs ranging from 18 to 30 nucleotides were used for library preparation. Amplification of these small RNAs was then accomplished by PCR. Next, the Illumina HiSeq 2500 platform was used to sequence these RNAs. We investigated the miRNA expression profiles of microglia-Exos using Illumina HiSeq 2500 high-throughput sequencing (miRNA-seq)