Source data.zip

This dataset supports the findings presented in the manuscript titled "Overcoming Radiotherapy Resistance in Uveal Melanoma through MDM2 Inhibition". The research investigates the role of SAR405838, an MDM2 inhibitor, in reversing radiotherapy resistance in uveal melanoma (UM) cells by targeting the MDM2-p53 axis.
Included Data:Raw Data:Cell viability assay (CCK-8) results in Excel format, comparing treated and untreated UM cells；Flow cytometry data (.fcs files) demonstrating apoptosis induction by SAR405838；Western Blot raw images showcasing MDM2 and p53 protein expression levels.Code and Analysis:R scripts for transcriptome sequencing differential gene expression and functional enrichment analysis；Data visualization scripts for generating heatmaps, volcano plots, and t-SNE clustering.Supporting Images:High-resolution microscopy and immunofluorescence images illustrating γ-H2AX expression.Transcriptome Sequencing:Differentially expressed gene tables and associated analysis results.Purpose and Relevance:This dataset provides insights into the molecular mechanisms underlying radiotherapy resistance in UM and demonstrates the therapeutic potential of MDM2 inhibitors in overcoming such resistance. It can be used by researchers working on targeted cancer therapies, radiotherapy optimization, or drug screening.
How to Use:Researchers can replicate the analysis using the provided raw data and scripts. For further information or inquiries, please contact [e-mail]