The age of the bone marrow microenvironment influences B-cell acute lymphoblastic leukemia progression via CXCR5-CXCL13 [ATAC-Seq]

We performed ATAC sequencing to determine if epigenetic changes regulate the function of young versus old macrophages. Young versus old BM macrophages were obtained by crushing femora, tibiae, and humeri of mice in phosphate-buffered saline (PBS) using mortar and pestle and then expanded in alpha-MEM medium supplemented with 20% fetal bovine serum, 1% penicillin/streptomycin and 1% L-glutamine, once non-adherent cells were removed. The immunophenotype of the adherent cells was confirmed by the following immunophenotype: F4/80+ CD169+.