Whole Genome Sequencing of Plasticizer-degrading Rhizobium pusense strain KSSKSLAB04

Rhizobium pusense strain KSSKSLAB04 is a plasticizer-degrading bacterium isolated from soil samples polluted with plastic waste collected from Juhu Beach, Mumbai, India. The strain was sequenced using a hybrid long-read strategy combining PacBio Onso and Oxford Nanopore PromethION platforms to generate high-quality genomic data. Genomic DNA was extracted from the isolate and processed to prepare libraries for both Onso and Nanopore sequencing using standard protocols. The integration of high-accuracy Onso short reads with long Nanopore reads enabled the construction of a highly contiguous draft genome assembly, supporting downstream genomic, functional, and environmental analyses of this plastic-degrading Rhizobium strain..Authors: Khushboo Sharma (Department of Bioinformatics, Alagappa University, Karaikudi - 630003, Tamil Nadu, India), Dr. Anuraj Nayarisseri (In Silico Research Laboratory, Eminent Biosciences, Indore - 452010, Madhya Pradesh, India), Prof. Sanjeev Kumar Singh (Department of Bioinformatics, Alagappa University, Karaikudi - 630003, Tamil Nadu, India)