Characterization of the Wheat-Psathyrostachys huashania 2Ns/2D Substitution Line H139: A Novel Germplasm with Enhanced Resistance to Wheat Take-all

Take-all is a devastating soil-borne disease that affects wheat production. The continuous generation of disease-resistance germplasm is an important aspect of the management of this pathogen. In this study, we characterized the wheat-Psathyrostachys huashania Keng-derived progeny H139 that exhibits significantly improved resistance to wheat take-all disease compared with its susceptible parent 7182. GISH) and mc-FISH analyses revealed that H139 is a stable wheat-P. huashania disomic substitution line lacking wheat chromosome 2D.EST-STS marker and Wheat Axiom 660K Genotyping Array analysis further revealed that H139 was a novel wheat-P. huashania 2Ns/2D substitution line, and that the P. huashania 2Ns chromosome shares high sequence similarity to wheat chromosome 2D. These results indicate that H139, with its enhanced wheat take-all disease resistance and desirable agronomic traits, provides valuable genetic resources for wheat chromosome engineering breeding. To further clarify the chromosome composition of H139, the Wheat Axiom 660K Genotyping Array was used for genotyping. A total of one sample (H139) was genotyped by wheat Axiom 660K Genotyping Array, which is due to the data of one sample enough to revealed that H139 was a novel wheat-P. huashania 2Ns/2D substitution line.