Purification of Encephalitozoon Cultures Contaminated by Mycoplasmas by Murine Intraperitoneal Inoculation
收藏PubMed Central2026-05-16 收录
下载链接：
https://pmc.ncbi.nlm.nih.gov/articles/PMC105057/
下载链接
链接失效反馈官方服务：
资源简介：
Encephalitozoon species are strict intracellular microsporidia. Cocultures with eukaryotic cell lines can become accidently contaminated by mycoplasmas. We propose a decontamination protocol based on differential cell targeting after intraperitoneal inoculation in mice. Mycoplasma-free microsporidia were isolated from the brains and spleens of inoculated mice 24 h postinoculation by using the centrifugation shell vial system. Identification was confirmed by direct sequencing of PCR-amplified 16S rRNA.
提供机构：
American Society for Microbiology (ASM)
