Comparison of the Illumina NextSeq2000 and GeneMind Genolab M for spatial transcriptomics

In this study, we compared two platforms Illumina NextSeq2000 and GeneMind Genolab M for 10x Genomics Visium spatial transcriptomics. The Illumina sequencing systems demonstrate high efficiency and power and remain the most popular platforms. Platforms with similar throughput and quality profiles but lower costs are under intensive development. Our analysis showed that GeneMind Genolab M sequencing platform generates consistent with Illumina NextSeq 2000 sequencing results. Sequencing metrics such as number of genes, cell barcodes, and unique molecular identifiers) as well as spatial distribution of spot clusters have the concordance between two platforms. Thus, GeneMind Genolab M instrument has similar to Illumina sequencing efficacy and is suitable for 10x Genomics Visium spatial transcriptomics.