BLASTN comparison of total nucleic acids, representative sequences from nucleic acid clusters and representative sequences from pORF clusters from this study and the Frias-Lopez study [9].

For each comparison two values are given, the first value is the percentage of Frias-Lopez data which is homologous to data from the current study; the second is the percentage of data from the current study which is homologous to the Frias-Lopez data. Comparisons were performed using BLASTN with the current studies dataset as reference database, and the Frias-Lopez dataset as the query. The (-b –v) parameter in BLASTN was set to 40,000. For every query sequence, every similar sequence in the reference dataset is identified. Sequences from both datasets that meet the criteria of an E-value <0.001 were included. Percentage values in parentheses are calculated by dividing each value by the total number of sequences/representative sequences for each dataset. For the Frias-Lopez data: Total DNA – 414,323, Total DNA nuc-clusters – 334,940, Total DNA pORF clusters – 390,599, Total mRNA – 128,234, Total mRNA (rRNA removed) - 63,111, Total mRNA nuc-clusters – 69,948, Total mRNA nuc-clusters (rRNA removed) - 43,948, Total mRNA-pORF clusters – 46,703. For the Gilbert data: Total DNA – 992,224, Total DNA nuc-clusters – 630,159, Total DNA pORF clusters – 1,083,644, Total mRNA – 506,353, Total mRNA nuc-clusters – 133,447, Total mRNA pORF clusters – 238,655. Percentage values in bold are normalised by divided each value through the Total DNA or Total RNA for the relevant study. Nuc-cluster refers to nucleotide clusters.