Perforin-2 (MPEG1) knockout mice are not resistant to LPS induced septic shock.

In this animal model LPS is injected to induce sterile septic shock. C57BL/6 and 129X1/SvJ wild-type and perforin-2 (mpeg1) knockout mice were injected intraperitoneally with LPS from <i>E. coli</i> 0111:B4 (InvivoGen LPS-EB ultra-pure, catalog # tlrl-3pelps). Mice were monitored hourly for signs of morbidity and humanely euthanized as per IACUUC protocol. "1" indicates subject death, "0" indicates censored subject. On either genetic background the differences between survival rates of WT and perforin-2 KO animals are insignificant. Crucially, we did observe that 129 mice were more resistant to LPS challenge than BL6 mice. This was expected due to the caspase 11 mutation carried by 129s. <i>All animal experiments were approved by and performed in accordance with the University of Miami Institutional Animal Care and Use Committee guidelines.</i><i><br></i><b>Experiment 1. </b>LPS challenge of WT and perforin-2 (mpeg1) -/- C57BL/6 and 129X1/SvJ mice<br><b>Experiment 2</b>. LPS challenge of WT and perforin-2 (mpeg1) -/- 129X1/SvJ mice

本动物模型通过注射脂多糖（lipopolysaccharide, LPS）诱导无菌性脓毒症休克。对C57BL/6及129X1/SvJ品系的野生型（wild-type, WT）与穿孔素2（mpeg1）敲除（knockout, KO）小鼠，予以腹腔注射大肠杆菌（E. coli）0111:B4来源的LPS（购自InvivoGen公司的LPS-EB超纯级，货号tlrl-3pelps）。实验小鼠每小时接受一次发病状态监测，并按照IACUUC规程实施人道安乐死。以“1”代表受试个体死亡，“0”代表受试个体截尾数据。在两种遗传背景下，野生型与穿孔素2敲除小鼠的生存率差异均无统计学意义。尤为关键的是，本研究观察到129系小鼠对LPS攻毒的耐受性显著高于BL6系小鼠，该结果符合预期，因129系小鼠携带半胱天冬酶11（caspase 11）突变。*所有动物实验均经迈阿密大学实验动物管理与使用委员会批准，并严格遵循其相关指南开展。*<br>**实验1：** 野生型与穿孔素2（mpeg1）敲除的C57BL/6及129X1/SvJ小鼠的LPS攻毒实验<br>**实验2：** 野生型与穿孔素2（mpeg1）敲除的129X1/SvJ小鼠的LPS攻毒实验