Genome-wide analysis of gene function in Botrytis strain HZ-011

To clarify the genomic information of Botrytis strain HZ-011 and to screen the relevant functional genes of strain HZ-011 for weed control, the whole genome of the DNA of Botrytis strain HZ-011 was sequenced in this study using the third-generation single-molecule sequencing technology. The splicing and assembly of the three generations of sequencing data were performed using Hifiasm software, the gene assembly results were predicted using GeneMarkS software, and the predicted gene protein sequences were compared with the four major databases for gene annotation using the BLASTTx program. The results showed that the total gene length of Botrytis strain HZ-011 was 4,402,230 bp, with 41.72% GC content. In total, 12,654 coding genes and 385 non-coding genes were predicted for this strain by whole genome sequencing. The basic annotation results showed that 12,250 genes were annotated, accounting for 96.88% of the total number of coding genes. The results of average nucleotide identity (ANI) analysis identified strain HZ-011 as Botrytis galanthina. The advanced genome annotation results showed that 1,629 genes were annotated in the CAZy database, and 1,864 genes were annotated in the PHI database, of which 54 genes were associated with the pathogenicity of pathogenic bacteria. In addition, 59 clusters of secondary metabolite synthesis genes and 12,654 signaling proteins were predicted, of which 1,088 secreted proteins were predicted, accounting for 8.6% of the total number of predicted signal peptide proteins. The genome-wide data of Botrytis galanthina strain HZ-011 provide a good foundation for in-depth studies of the pathogenicity-regulating genes of this strain and for investigating the pathogenicity mechanism of this strain.